Organic Compound # 2

Experiment # 9 Testing for Protein

Materials Needed:
* Protein chemical indicator (see below for details)
* Flour
* cheesecloth
* bouillon
* Dextrostik strips  or Cinitest (to test sugar)
* Iodine (to test carbohydrates)
* a variety of food

Chemical Indicator : a mixture of household ammonia with a few drops of 3% copper sulfate solution

Procedure:
1) Add some chemical indicator mixture to milk. What color do you observed?

L-carbo test; R-protein (#4,5)

2) Dissolve a bouillon cube in hot water and test with protein chemical indicator. What did you observe?
3) Obtain a cup of flour and mix with water to form a stiff paste. Knead for about 10 minutes, put into a cheesecloth and continue kneading under water. Continue rinsing until the water becomes milky.
4) Test the water for carbohydrates. What were your results?
5) Test the water for protein. What were your results?
6) Test a portion of the residue in the cheesecloth for carbohydrate. What were your results?
7) Test the residue for protein. What were your results?
8) Discuss results in steps 4, 5, 6 and 7.
9) Try various foods to see if they contain protein.
10) Write a summary of your results of this experiment.

Top: R- meat tested for protein; Below: L-residue tested, R- fruits tested

Findings:
* Soluble starch from flour paste dissolves in water
* Insoluble residue left in the cheesecloth is protein
* Meat has protein
* Fruits tested do not have protein

Organic Compounds #1

Experiment #8 : Testing for Sugar and Starch

Materials Needed:
* Powdered Starch
* Beakers or tumblers
* several fruits and vegetables
* Dextrostik or Clinitest (to test sugar)
* Iodine
* Sugar (Karo syrup)

Procedure:
1) Put a teaspoonful of starch in one beaker of water and a teaspoonful of sugar in a second beaker of water. Stir both solutions.
2) Test for starch with Iodine and sugar with Clinitest tablets or Destrostik strips.
3) Test several fruits and vegetables for starch and sugar.

L-R: Sugar; starch

Testing for Starch L-R: potato, apple, pineapple, carrots

Testing for Sugar L-R: apple, pineapple, carrots, potato

Findings:
* The beaker with starch turned blue.
* The strip turned green when dipped in the beaker with sugar.
* Potato, carrots, apple turned blue when tested with iodine. Pineapple had no reaction.
* Potato and apple showed changed color on their respective strips. While carrots and pineapple did not.

We are almost ready. . . for Science

Made that trip to Sta. Cruz, Manila last week to scour for our Science experiment materials.

Our stash is almost complete for Unit 2 and we are ready to go! Gia and I can't wait to start with our Science experiments and activities. =)

The list of materials ( excluding common household items) for AOP Lifepac Science for Grade 10 consists of:

• Alligator clip leads, 2/pk
• Alpha Omega Biology Slide Set

  Ascaris mitosis, onion root tip, Diatoms, frog ovary, frog sperm, human blood smear, human bone, kidney section, muscle types section, motor neuron smear, Spirogyra, and Ulva microscope slides

• Battery, 6 volt, alkaline
• Beaker, glass, 250 ml
• Biuret reagent, 30 ml
• Calcium hydroxide, 30 g (lime)
• Chalk (calcium carbonate), 12/pack
• Cups & lids, 8 oz plastic, 3/pack
• Electrolysis electrodes (2)
• Filter paper, 10/pkg
• Forceps, stainless steel
• Funnel, plastic, 65 mm dia.
• Glucose test strips, 50 pack
• Graduated cylinder, plastic, 10 ml
• Hydrochloric acid, 30 ml
• Iodine solution (Lugol's) 30ml
• Lab apron, economy
• Litmus paper, blue, 100 strips
• Litmus paper, red, 100 strips
• Magnifier, tripod, 10X
• Methylene blue, 1%, 15 ml
• Osmosis Set
• P.T.C. test paper, 100/vial
• Petri dish, plastic, 2/pk
• Phenolphthalein solution, 15ml
• Pipet (medicine dropper)
• Potassium permanganate, 30 g
• Probe & seeker, chrome, 6"
• Protozoa Hatchery Kit
• Razor blade, single edge
• Safety gloves, size 8-8½ med.
• Safety goggles, full-size
• Sand, coarse, 1 lb.
• Scissors, dissecting, 4½"
• Screen, 20 mesh, steel, 4x4"
• Seeds, grass, 6 g pack
• Seeds, lima bean, 15 g pack
• Seeds, marigold, 300 mg pack
• Seeds, radish, 4 g pack
• Basic microscope slide set: 12 plain slides, 2 concave slides, coverslips, and storage box
• Sodium sulfate, 30 g
• Stirring rod, glass, 6"
• Teasing needle, straight
• Test tube rack, 6 holes
• Test tubes, small 13x100 mm
• Test tubes, x-large 19x150 mm
• Thermometer, Celsius, 12"

Yes! a very long list of items that. . . after the school year, we will have our own Science Laboratory! LOL!

Hopefully we will be able to complete them before the end of the school year. One Unit at a time. :)

Unit 2 (Basis of Life) will be about : 

* molecular models
* ionic and covalent bonding
* inorganic compounds experiment about solvent, thermal properties of water, acids,  bases and salts
* chemical reactions like electrolysis and using yeasts
* organic compounds like testing for starch, sugar, oil and protein in some food
* and,  properties of enzymes

So here's our stash (in photo) :

Grade 10 Unit 2 materials

stored securely (p.s. we need a bigger container soon)

Oh how this simple exercise of gathering all the materials reminds me of how I love Science back when I was in school. 

Rocking Science we should be!